Composite
ALPaGA Col

Part:BBa_K4782006:Design

Designed by: Adina Kadyrova   Group: iGEM23_NU-Kazakhstan   (2023-10-11)

We used two biobrick: BBa_K4244000 (ALPaGA, iGEM22_Wageningen_UR) and BBa_K2922024 (Colicin E1+immunity protein, GEM19_XMU-China). ALPaGA (A Lactate Promoter Operating in Glucose and Anoxia) is the codon-optimized part based on the wild-type promoter from lldPRD operon (BBa_K1847008)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1535
    Illegal AgeI site found at 1592
    Illegal AgeI site found at 1724
  • 1000
    COMPATIBLE WITH RFC[1000]